Cation contents are expressed in micromoles per gram of dry cell solids. □, Na+ content; , K+ content. Control refers to uninjected oocytes. No difference could be detected between water-injected and uninjected oocytes. The bars represent means ±s.e.m.; n = 3. A, cation contents of control oocytes at time zero and 5 days later compared to tAE1- or mAE1-injected oocytes maintained 5 days after cRNA injection in a ouabain-free, normal Cl−-containing saline (MBS). B, cation contents of mAE1- or tAE1-injected oocytes maintained only 2 days after cRNA injection in MBS containing 10−4m ouabain compared to tAE1-injected oocytes maintained for two days in MBS without ouabain. C, cation contents of control and tAE1-injected oocytes maintained 2 days after cRNA injection in a Cl−-free, NO3−-containing saline (NO3-MBS) in the presence of 10−4m ouabain and 10−6m bumetanide.