Figure 3. Effects of inhibitors on tAE1-induced Rb⁺ (A) and Li⁺ influxes (B) and on the changes in Na⁺ and K⁺ contents (C).

Influxes of Rb⁺ (A) and Li⁺ (B) in tAE1-expressing oocytes were measured at day 5 post-injection in a Cl⁻-free, NO₃⁻-containing saline (NO₃-MBS for Rb⁺ influx or Li-MBS for Li⁺ influx) in the presence of ouabain 10⁻⁴m (control) and the following inhibitors: bumetanide, 10⁻⁶m; NPPB, 10⁻⁴m; NAP-taurine, 10⁻³m, or niflumic acid, 10⁻⁴m. Bars represent means ±s.e.m.; n = 8. C, □, Na⁺ content; , K⁺ content. tAE1-injected oocytes were maintained 2 days after cRNA injection in Cl⁻-containing saline (MBS) in the presence of ouabain 10⁻⁴m and bumetanide 10⁻⁶m and either NPPB, 10⁻⁴m, or niflumic acid, 10⁻⁴m. Bars represent means ±s.e.m.; n = 3. Control refers to uninjected oocytes maintained for 2 days in MBS with 10⁻⁴m ouabain and 10⁻⁶m bumetanide.