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. 2001 Sep 1;535(Pt 2):497–506. doi: 10.1111/j.1469-7793.2001.t01-1-00497.x

Figure 8. Covalent H2DIDS treatment and transport properties of the mutant K522R.

Figure 8

In K522R, the Lys-522 has been converted to Arg by site-directed mutagenesis, thus preventing covalent binding of H2DIDS. □, K522R; ▪, K522R in presence of 5 × 10−5m H2DIDS; Inline graphic, covalent binding of H2DIDS to K522R. Measurements at day 5 post-injection. Bars represent means ±s.e.m.; n = 8. A, chloride exchange activity; B, anion conductance; C, lithium influx.