Figure 1. Sample single-channel clusters and open and closed time histograms for the wild-type, hybrid and mutant αY93F receptors.

The receptors were activated by 500 μm ACh. Pipette potential was +50 mV. Inward current is shown downward. A, wild-type receptor (WT). B, hybrid receptor (H). C, mutant receptor (MT). For the mutant receptor (C), only a portion of a cluster is shown. Note also that a different time scale has been used for the mutant receptor cluster. Continuous lines in histograms were calculated according to the rate constants shown in Table 2.