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. 2001 Nov 15;537(Pt 1):57–68. doi: 10.1111/j.1469-7793.2001.0057k.x

Figure 1. HEK 293 cells express functional glucocorticoid receptors.

Figure 1

A, whole cell lysates (10 μg protein from AtT20 cells as positive control and 50 μg from HEK 293 cells) were separated by SDS-PAGE and transferred to PVDF membranes. Blots were probed as described in methods with an anti-GR (M20) rabbit primary antibody that recognises both GRα and GRβ isoforms with detection by ECL; ∼95 kDa immunoreactive bands were detectable in both AtT20 and HEK 293 cell lysates. B, functional assay of glucocorticoid receptor activation in HEK 293 cells using a MMTV-luciferase reporter assay. HEK 293 cells were transiently transfected with 1 μg of MMTV-luc plasmid (○) or pcDNA3 vector alone (•) and treated for 2 h with various concentrations of the synthetic glucocorticoid receptor agonist dexamethasone or vehicle before assay for luciferase activity as described in Methods. Data are expressed as a percentage of maximal (with 1 μm dexamethasone) luciferase activity after vehicle background subtraction. Each data point represents the mean ± s.e.m (n = 4–8).