Figure 4. Time course of the effect of i.t. instillation of PLL on pulmonary C fibre responses to lung inflation.

A, responses to lung inflation at low pressure (P_t= 15 cmH₂O); B, responses to lung inflation at high pressure (P_t= 30 cmH₂O). ΔFA was measured as the difference between the 10 s average during lung inflation and the 10 s average at baseline in each fibre. The responses were determined at 10 min before, and 10, 30, 60 and 120 min after i.t. instillation of a low dose of PLL (25 μg in 0.1 ml, ; n = 8), a high dose of PLL (50 μg in 0.1 ml, ▪; n = 16) and vehicle control (□; n = 6). Data are means ±s.e.m. *P < 0.05, significant difference between vehicle and 25 or 50 μg of PLL at any time point; §P < 0.05, significant difference between 25 and 50 μg of PLL at any time point; †P < 0.05, significant difference between before and any time point after instillation of PLL or vehicle.