. 2001 Dec 15;537(Pt 3):659–665. doi: 10.1111/j.1469-7793.2001.00659.x

Table 1.

Effects of PKA modulators on reversal potential (E_rev) and stoichiometry (n) of recombinant kNBC1 clones in mPCT cells

	WT kNBC1		EGFP-WT-kNBC1		S982A-kNBC1		EGFP-S982A-kNBC1

[Na⁺]_AP/[Na⁺]_BL	E_rev	n	E_rev	n	E_rev	n	E_rev	n
No treatment
10/50	−20.1 ± 2(6)	3.1 ± 0.3	−19.4 ± 2(4)	3.2 ± 0.3	−18.6 ± 2(7)	3.2 ± 0.3	−19.4 ± 2(6)	3.2 ± 0.3
50/10	23.2 ± 2(5)	2.8 ± 0.3	21.5 ± 2(4)	2.9 ± 0.3	19.1 ± 2(8)	3.2 ± 0.3	20.3 ± 2(4)	3.1 ± 0.3
8-Br-cAMP
10/50	−38.2 ± 4(7)^*	2.1 ± 0.2^*	−37.6 ± 4(6)^*	2.1 ± 0.2^*	−22.4 ± 2(6)	2.9 ± 0.3	−21.3 ± 2(4)	3.0 ± 0.3
50/10	37.5 ± 4(8)^*	2.1 ± 0.2^*	44.2 ± 4(5)^*	1.9 ± 0.2^*	18.6 ± 2(7)	3.2 ± 0.3	23.1 ± 2(5)	2.8 ± 0.3
H-89 + 8-Br-cAMP
10/50	−23.7 ± 2(8)	2.8 ± 0.3	−22.6 ± 2(4)	2.8 ± 0.3	−18.8 ± 2(7)	3.2 ± 0.3	−20.4 ± 2(6)	3.1 ± 0.3
50/10	22.4 ± 2(5)	2.9 ± 0.3	24.1 ± 2(6)	2.7 ± 0.3	22.9 ± 2(6)	2.8 ± 0.3	23.5 ± 2(4)	2.8 ± 0.3
H-85 + 8-Br-cAMP
10/50	−35.2 ± 3(4)^*	2.2 ± 0.2^*	−36.6 ± 2(4)^*	2.1 ± 0.2^*	−24.1 ± 2(4)	2.7 ± 0.3	−21.6 ± 2(4)	2.9 ± 0.3
50/10	37.4 ± 3(4)^*	2.1 ± 0.3^*	34.3 ± 2(4)^*	2.2 ± 0.2^*	23.2 ± 2(4)	2.8 ± 0.3	22.5 ± 2(4)	2.8 ± 0.3

[Na⁺]_AP/[Na⁺]_BL, apical/basolateral sodium gradient. Number of experiments is shown in parentheses.

P < 0.05 compared with the corresponding WT kNBC1 construct in untreated cells.