Figure 6. Time course of recovery of the response to GABA application in day experiments.

A, 4 superimposed current traces obtained during subjective day, each showing the response to the train of GABA pulses, followed by a short GABA test pulse delivered at different intervals. Membrane potential was held at either -40 mV (upper trace) or -70 mV (lower trace). B, time course of recovery from chloride accumulation (•) or depletion (○) for the results shown in A. Data are presented as the ratio between the amplitude of the test response and the amplitude of the first response. C and D, another experiment as described in A and B, but in this example the two recovery processes follow a similar time course. E and F, the recovery process is regulated by active mechanisms. E, the recovery process from chloride loading (upper traces) and chloride depletion (lower traces) at an ambient temperature of 26 °C (black traces) and 28 °C (grey traces). Note the 24 % increase in the amplitude of the response to the test pulse at the higher temperature. F, recovery at 30 s delay before (black traces) and after (grey traces) 1 mm furosamide application. Note the 40 % decrease in the test response amplitude in the recovery from chloride depletion and the 48 % reduction in the recovery from chloride loading.