Fig. 4. Top views reveal 6-fold symmetry.

Select class averages of (top) ΔN-Vps4p, (middle) full-length Vps4p, and (bottom) Vta1p-Vps4p complexes are shown. All the raw images were iteratively classified based on their similarity using singular value decomposition by refine2d.py. Particles within the same class were aligned and averaged to generate 50 class averages. The 18 class averages most closely corresponding to “top” views are shown, all of which suggest 6-fold symmetry.