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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Dev Cell. 2008 Mar;14(3):331–341. doi: 10.1016/j.devcel.2007.12.007

Figure 4.

Figure 4

sSgo1 functions in regulating spindle pole integrity. (A) The intronic sequences surrounding human exon 6 or its equivalent in other mammals were aligned. Red color marks the invariant splice donor and splice acceptor sequences. The shaded areas indicate non-conserved sequences in introns. (B) HeLa cells transfected with GFP-Sgo1 or GFP-sSgo1 expression constructs for 24 h were fixed and stained with antibodies to GFP (green), CREST (red, upper panel), or Plk1 (red, lower panel). (C) HeLa cells transfected with Myc-Sgo11-196 expression plasmid for 24 h were stained with antibody to Myc. (D&E) HeLa cells transfected with Myc-Sgo11-196 plasmid for 24 h were stained with antibodies to Myc (green) and γ-tubulin (red) (D). Various abnormal spindle pole patterns were summarized from three independent experiments (E) (150 mitotic cells/experiment). (F) HeLa cells expressing GFP-centrin transfected with Myc-Sgo11-196 plasmid for 24 h were stained with antibodies to Myc (red) and GFP (green). Arrows indicate the position of split centrioles.