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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Dev Cell. 2008 Mar;14(3):331–341. doi: 10.1016/j.devcel.2007.12.007

Figure 5.

Figure 5

Ectopic expression of sSgo1 suppresses centriole splitting induced by Sgo1 depletion. (A) HeLa cells co-transfected with the Myc-sSgo1 expression construct and Sgo1 3′UTR siRNA for 48 h were collected and an equal amount of cell lysate was blotted for Myc, endogenous sSgo1, and β-actin. (B & C) HeLa cells transfected with the Myc-sSgo1 expression construct and Sgo1 3′UTR siRNA for about 32 h were fixed and stained with antibodies to the Myc tag and γ-tubulin. The data were summarized from over 200 transfected mitotic cells. (D) Wild-type (Sgo1+/+) and Sgo1+/− MEF cells were stained with antibody to γ-tubulin (green). (E) The number of mitotic cells with extra γ-tubulin foci in Sgo1+/+ and Sgo1+/− MEF cells was scored. The data were summarized from three independent experiments (100 mitotic cells/experiment). (F) Sgo1+/− MEFs transfected with a Myc-sSgo1 expression construct for 24 h were stained with antibodies to γ-tubulin and the Myc tag. The number (n) of mitotic cells with extra γ-tubulin foci was scored (2 independent experiments). * denotes significant statistical difference (p<0.01).