Figure 3.

Effect of overexpression of various constructs on calpain concentration in transfectants. Myoblasts were cultured to 90–100% confluence, after which IPTG (5 mM) was added to each plate. The plates (except lane a) were cultured for an additional 8 h (lane b), 1 day (lane c), 2 days (lane d), 3 days (lane e), 4 days (lane f), or 5 days (lane g). Western blots were prepared and polyclonal rat m-calpain antibody and polyclonal LacI antibody were used to probe the blots as described in the text. (Top) L8/PC cell line; (Middle) L8/CID cell line; (Bottom) L8/DN cell line.