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Inhibition of fodrin-breakdown product by reduction of calpain activity in various cell lines. (Upper) Fodrin degradation in L8/PC cell line: no A23187 treatment (lane a), A23187 (10 μM) for 1 h (lane b), and A23187 (20 μM) for 2 h (lane c). (Lower) Fodrin degradation in the presence and absence of IPTG in the cell lines L8/CID and L8/DN exposed to A23187 (20 μM) for 2 h. Monoclonal spectrin antibody (nonerythroid) was used for Western blotting.
