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Stabilization of nebulin by reduction of calpain activity. The three cell lines (L8/PC, L8/DN, and L8/CID) were exposed to IPTG (5 mM) for 24 h, after which nebulin concentration was assessed by Western blotting. Lanes a and b are duplicates, as are lanes c and d. Nebulin was stabilized by 20% after overexpression of DN-m-calpain and 33% after overexpression of CID.
