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. 2008 Mar 19;2008:317851. doi: 10.1155/2008/317851

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Copyright © 2008 Vitaliy Poylin et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sepsis-induced expression of MuRF1 in extensor digitorum longus muscles is not influenced by curcumin. Sham-operated and septic rats were treated with vehicle (control) or curcumin (total dose 1800 mg/kg divided into three equal doses administered intraperitoneally 1 hour before and 8 and 15 hours after sham-operation or CLP). Muscles were harvested 16 hours after sham-operation or CLP for (a) MuRF1 mRNA levels determined by real-time PCR and (b) MuRF1 protein levels determined by Western blotting. In panel (b), a representative Western blot is shown in the upper portion and results from densitometric quantifications are shown in the lower portion of the figure. Results are means ± SEM with n = 8 in each group. AU: arbitrary units. *P < .05 versus corresponding sham group by ANOVA.