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. Author manuscript; available in PMC: 2009 Feb 26.
Published in final edited form as: Eur J Pharmacol. 2007 Nov 28;581(1-2):19–29. doi: 10.1016/j.ejphar.2007.11.053

Fig. 3. Enrichment of the fully glycosylated form (64 kDa) of human cannabinoid CB1- myc-His6 receptor by lectin affinity chromatography and gradient elution.

Fig. 3

The figure shows a representative Western blot probed with an antibody raised against the N-terminus of the human cannbinoid CB1 receptor. 1. solubilized Sf9/ human cannabinoid CB1- myc-His6 receptor membrane preparation. Glycosylated proteins were eluted from the WGA column with increasing concentrations of 2. 0.125 M, 3. 0.25 M and 4. 0.5 M N-acetylglucosamine; 5. molecular weight markers.