Fig. 6. The human cannabinoid 1- myc-His6 receptor does not co-purify with insect G proteins from infected Sf9 cells.
A representative Western blot of the 1. solubilized and 2. partially purified human cannabinoid CB1- myc-His6 receptor preparations, probed with a nonselective anti-G protein α-subunit antibody (A569). Equal amounts of 2 μg total protein have been loaded on lanes 1 and 2.