Table 1.

Numbers of plants exhibiting identical transgene integration patterns detected among segregating transgene-positive progeny within a line indicating the presence of single transgene loci

Line	Introduced plasmids	No. of plants analyzed
		Transgenepositive plants	Transgenenegative plants
300	pBARGUS	26	4
301	pBARGUS	45	35
503	pBARGUS + pH24	6	2
504	pBARGUS + pH24	25	6
601	pBARGUS + pRPV*	25	8
607	pBARGUS + pMAV	13	16
803	pBARGUS + pMAV	13	2
804	pBARGUS + pPAV	14	7
903	pBARGUS + pRPV	5	10
904	pBARGUS + pPAV	4	5
909	pBARGUS + pRPV	3	2
1010	pBARGUS + pMAV*	4	2
1017	pBARGUS + pMAV*	3	2

Southern blots were probed with bar, gusA, the entire pBARGUS sequence, and the coding regions of the three BYDV coat protein genes. To detect unlinked transgene fragments with a probability greater than 0.95, 11 transgene-positive T₁ individuals or 7 transgene-positive T₂ individuals need to be analyzed (8 and 5, respectively, for P > 0.90). Alternatively, analysis of 3 transgene-negative T₁ or 4 transgene-negative T₂ plants is sufficient for P > 0.95 (2 and 3, respectively, for P > 0.90). T₁ and/or T₂ plants were sampled based on the presence or absence of transgene phenotypes. Thus, the ratio of transgene-positive to transgene-negative plants does not reflect the transgene segregation ratio in the line. 

^*BYDV coat protein gene was not detected in the transgenic plants nor in the progenitor tissue culture.