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. 2008 Apr 1;22(7):931–944. doi: 10.1101/gad.1648308

Figure 2.

Figure 2.

CR and the NADH shuttle system function in the same pathway. (A) CR does not synergize with the overexpressions to extend life span. Life span analysis of cells overexpressing Mdh1, Aat1, and Gut2 grown under normal and CR (0.5% glucose) conditions. (B) Deletions of single components of the NADH shuttle systems partially inhibit CR-mediated life span extension. (C) Complete inactivation of the malate–aspartate shuttle totally abolishes CR-induced life span extension. (D) CR (0.5% glucose) is sufficient to induce expression of the shuttle component. Western blot analysis of the Mdh1 protein levels in cells grown in 2% and 0.5% glucose. Mdh1 is fused to an HA epitope tag and visualized with the anti-HA antiserum. (WT) Wild-type control; (oe) overexpression. One set of representative data is shown.