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. 2008 Apr 1;22(7):931–944. doi: 10.1101/gad.1648308

Figure 4.

Figure 4.

CR genetic models and the NADH shuttle. (A) Overexpression of Hap4 requires the malate–aspartate shuttle for life span extension. Life span analysis of the wild-type and Hap4-oe cells, the mdh1Δaat1Δ double mutant, and gut2Δ single mutant with and without Hap4-oe on 2% glucose media. (B) The cdc25-10 mutation requires the malate–aspartate shuttle for life span extension. Life span analysis of the wild-type and cdc25-10 mutant cells, the mdh1Δaat1Δ double mutant, and gut2Δ single mutant with and without the cdc25-10 mutation on 2% glucose media. (C) Lat1 overexpression requires both the malate–aspartate shuttle and the glycerol-3-phosphate shuttle to extend life span. (D) Mdh1 overexpression requires the glycerol-3-phosphate shuttle to extend life span and Gut2 overexpression requires the malate–aspartate shuttle to extend life span. (WT) Wild-type control; (v) empty vector control; (oe) overexpression. One set of representative data is shown.