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. 2008 Feb 29;4(2):e1000007. doi: 10.1371/journal.ppat.1000007

Figure 4. TRIM22 expression blocks release of HIV-1 Gag-only particles.

Figure 4

A) Cells were transfected with pGag and pTRIM22 or pcDNA. Cells and Gag-only particles released into the supernatant were isolated 24 hours after transfection and analyzed by Western blotting using an anti-p24CA or anti-actin antibody. B) Cells were pre-treated for 16 hours with 1000 units/ml of IFNβ followed by transfection with pGag. Cells and Gag-only particles released into the supernatant were isolated after 24 hours and analyzed by Western blotting using an anti-p24CA antibody. C) Cells were co-transfected with pR9 or a plasmid expressing MLV GagPol (pMLV) or an EIAV packaging vector (pEIAV) with or without TRIM22 for 24 hours. Cells and Gag-containing particles were isolated and analyzed by Western blotting using an anti-capsid antibody.