Figure 3.

Sequence alignment, secondary structure elements, and interactive sequences for human αB crystallin, sHSP16.5, and sHSP16.9. The observed secondary structure of wheat sHSP16.9 and Methanococcus jannaschi sHSP16.5 along with the predicted secondary structure of human αB crystallin (~,α helix;-, β strand) are shown. The interactive sequences in the dodecameric quaternary structure of wheat HSP16.9 are in green boxes; the interactive sequences for the Mj sHSP16.5 24-mer structure are in blue boxes. The interactive domains identified in human αB crystallin using protein pin arrays are in orange boxes and corresponded with the interactive sequences observed in the crystal structures of Mj sHSP16.5 and wheat sHSP16.9. In the crystal structure of wheat sHSP16.9, the α helix in the N terminus interacted with β4 and β7 in the α crystallin core domain.