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HSQC map of LD4 titration into a solution containing the FAT domain. (A) Overlaid HSQC spectra of the FAT domain (red), FAT with 1 eq of LD4 (orange), and FAT with 10 eq of LD4 (green). (B) Comparison of the HSQC spectra of FAT with 1 eq of LD2 (blue) and with 1 eq of LD4 (red). The difference in the spectra indicates that the two peptides bound to different sites in FAT.
