Fig. 4.
β-Catenin mRNA expression was correlated with cyclin D1, c-myc and c-jun mRNA levels in DMH-induced tumors. qPCR was used to examine DMH-induced tumors and normal mucosa for expression of three reported β-catenin/Tcf downstream targets, as well as AP-2α, a negative regulator of β-catenin/Wnt signaling [20]. In each case, mRNA expression was first normalized to the housekeeping gene, GAPDH. Open symbols (○), tumors containing WT β-catenin; closed symbols (●), tumors with mutant β-catenin.
