Table 2.

Kinetic refolding parameters for wild-type and mutant HγD-Crys

Protein	k1 (sec−1)	t1/2 (sec)	k2 (sec−1)	t1/2 (sec)
Wild typea	0.048 ± 0.001	15 ± 1	0.0037 ± 0.0001	190 ± 10
R79A	0.037 ± 0.006	19 ± 3	0.0008 ± 0.0001	890 ± 50
M147A	0.032 ± 0.002	21 ± 1	0.0010 ± 0.0001	680 ± 20
Q54A	0.040 ± 0.001	17 ± 1	0.0023 ± 0.0002	310 ± 30
Q143A	0.041 ± 0.003	17 ± 1	0.0016 ± 0.0001	430 ± 10
R79A/M147A	0.030 ± 0.004	23 ± 3	0.0004 ± 0.0001	1700 ± 20
Q54A/Q143A	0.033 ± 0.004	20 ± 2	0.0012 ± 0.0001	600 ± 40

^aFrom Flaugh et al. (2005).