Figure 2.

Expression of fluorescent colonies. (A) Example library plate irradiated with 366 nm UV light. Approximately 1% of colonies show strong fluorescence. (B) Examples of colonies selected for expression tests. Colonies 1–10 are brightly fluorescent while colony 11 represents a control colony that is not fluorescent. Fluorescence was visualized after exposing the plate to 366 nm UV light. (C) SDS-PAGE analysis of GFP-fusion protein expression of selected colonies. Asterisks are located to the left of the band representing overexpressed GFP-fusion proteins in each lane. Numbers above each lane correspond to the colony shown in B. Numbers to the left indicate protein molecular weight markers in kDa.