Table 1.
Regions of T. thermophila TERT identified as expressed and soluble by high-throughput screening
Clone | Residues | Mutations |
1 | 1–268 | |
2 | 1–201 | |
3 | 1–192 | |
4 | 1–112 | |
5 | 133–237 | |
6 | 137–371 | E370Q |
7 | 128–370 | |
8 | 162–283 | V169A |
9 | 200–394 | N202S |
10 | 199–388 | |
11 | 208–382 | |
12 | 277–372 | P278L, D279E, H280R, Y367H, D368A, I371P |
13 | 321–390 | |
14 | 301–435 | |
15 | 464–709 | |
16 | 470–703 | Q702K |
17 | 482–578 | |
18 | 484–701 | |
19 | 484–704 | Q702P |
20 | 493–781 | |
21 | 493–783 | C782Y |
22 | 551–773 | S753G |
23 | 550–737 | K736N |
24 | 664–772 | N666D, S753G |
25 | 673–782 | |
26 | 711–782 | |
27 | 709–781 |