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. 2005 Aug;14(8):2087–2094. doi: 10.1110/ps.051501605

Table 2.

Steady state kinetic analysis of S. aureus FabH

Acyl-CoA Km (acyl-CoA) (μM) kcat (min−1) kcat /Km (M−1min−1 × 106)
Isobutyryl-CoA 37 ± 6 1682 ± 43 45
Hexanoyl-CoA 13 ± 2 1528 ± 84 118
Butyryl-CoA 20 ± 2 1254 ± 48 63
Isovaleryl-CoA 13 ± 2 930 ± 47 72
Acetyl-CoA 373 ± 17 261 ± 34 1

Substrate concentrations for apparent Km determinations for S. aureus FabH were as follows: 30 μM malonyl-ACP,5–300 μM acyl-CoA, 100 μM NADPH, 1.5 μg S. pneumoniae FabG, and 0.01 μg S. aureus FabH.