Figure 1.

SDS-PAGE analysis of expression levels in E. coli and solubilized inclusion body preparations of HAT-hβ₂m. (A) Expression level of HAT-hβ₂m before and after induction. (Lane 1) Protein marker; (lane 2) before induction; (lanes 3–5) expression levels after 1, 2, and 3 h of induction, respectively. (B,C) Reducing and nonreducing SDS-PAGE analysis of feedstock A. (Lane M) Protein marker. DTT, dithiothreitol; 2-ME, 2-mercaptoethanol. Molecular weights of standard protein are indicated, and the positions of reduced (0) and oxidized (1) HAT-hβ₂m monomers are shown with arrows.