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. 2005 Aug;14(8):2177–2186. doi: 10.1110/ps.051409205

Figure 4.

Figure 4.

TM5 peptide self-association monitored by FRET. (A) TM5W tryptophan fluorescence emission spectrum (310–500 nm) upon excitation at 295 nm in the presence or the absence of TM5Pyr: 10 μM TM5W (solid line) and 20 μM (TM5W+TM5Pyr) (dashed line). (B) TM5Pyr excimer fluorescence emission spectrum (360–550 nm) upon excitation at 345 nm in the presence or the absence of TM5W: 10 μM TM5Pyr (solid line) and 20 μM (10 μM TM5W+10 μM TM5Pyr) (dashed line). For ease of comparison, intensities of the spectra are normalized to the 380 nm peak of the pyrene monomer. Total absorbance in all cases was <0.1 at the excitation wavelength to avoid inner filter effects.