. 2005 Aug;14(8):2187–2194. doi: 10.1110/ps.051405005

Table 1.

The Stokes radii (in Ångstroms) of nonmodified and glucosylated b-LGA as determined by size exclusion chromatography and fluoresence correlation spectroscopy

	Nonmodified		Glucosylated
	GPC^a	FCS^b	GPC^a	FCS^b
0 M	27.0	30.0	27.9	30.9
8 M	35.7	36.8	36.1	36.2

^aBased on calibration of the size exclusion column using a set of standard proteins with reported Stokes radii. R²-value of calibration line (elution volume vs. Stokes radius) was 0.996. Accuracy of established radii was ± 0.3Å.

^b The Stokes radius was calculated according to r=kT/(6πηD), where D (diffusion coefficient) was calculated using x² =2Dt, with x being the radius of the selected volume (0.22 μm) and t the observed diffusion time. The intrinsic viscosity was corrected for the contribution of 8 M urea according to the standard formula given by Kawahara and Tanford (1966). The estimated accuracy of the values was ± 0.4 Å.