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Translocation of RepA from ClpA to inactive ClpP. [3H]RepA (34 pmol), ClpA (28 pmol), and chemically inactivated ClpP (11 pmol) were incubated first with ATP[γ-S] and then with ATP. Samples were then treated with 1 M NaCl and ClpP antibody. Coimmunoprecipitated RepA was quantitated by measuring the amount of radioactivity in the recovered immunoprecipitates. Control experiments were performed as indicated. Results are means (±SEM) of two to five independent experiments.
