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Translocation of RepA from [3H]ClpA to inactivated ClpP. Experimental conditions were identical to those described in Fig. 4. In experiment 1 [3H]RepA and unlabeled ClpA were used, whereas in experiments 2–5 [3H]ClpA and unlabeled RepA were used. Control experiments with added or omitted components are as indicated. In the absence of added ClpP and RepA, 0.02 pmol of [3H]ClpA associated with the immunoprecipitates and has been subtracted.
