Table 2.

Specificity of ATP-dependent substrate translocation from ClpA to chemically inactivated ClpP

Experiment	Additions to first reaction	RepA associated with ClpP immunoprecipitates, pmol
1	Complete: [3H]RepA, ClpA, ClpP (inactivated), ATP[γ-S]	1.09 ± 0.11
2	Complete plus 20-fold molar excess of RepA to [3H]RepA	0.05 ± 0.03
3	Complete plus 20-fold molar excess of casein to [3H]RepA	0.17 ± 0.01
4	Complete plus 20-fold molar excess of lysozyme to [3H]RepA	1.09 ± 0.24
5	Complete plus 20-fold molar excess of ovalbumin to [3H]RepA	0.87 ± 0.01
6	Complete, with a 20-fold molar excess of RepA to [3H]RepA added after incubation with ATP	1.14 ± 0.22

Translocation experiments were identical to those described in Fig. 4, but for experiments 2–5 a 20-fold molar excess of unlabeled RepA, α-casein, lysozyme, or ovalbumin was added during the first incubation step as indicated. In experiment 6, a 20-fold molar excess of unlabeled RepA was added after the ATP incubation step. Results are means (±SEM) of two independent experiments.