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Kinetic parameters determined for the reassembled mDHFR
| Km(NADPH), μM | Km (DHF), μM | kcat, s−1 | Ki (MTX), nM | |
|---|---|---|---|---|
| Reassembled mDHFR (Phe-31 → Ser) | 110 | 11.1 ± 0.4* | 3.2 ± 1.9 | 0.54 ± 0.33 |
| Full-length mDHFR (Phe-31 → Ser) (ref. 21) | 33 | 4.5 | 5.5 | 4.4 |
Values given as the mean ± SD for the results of two to five experiments.
