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. Author manuscript; available in PMC: 2009 Mar 21.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jan 14;367(4):852–858. doi: 10.1016/j.bbrc.2008.01.016

Fig. 1. Absorption spectral changes of the heme-rat HO-1 complex during the NADPH/CPR-supported single-turnover reaction.

Fig. 1

The spectra were recorded before (dotted line; ········) and after the addition of NADPH; 5 (dashed and dotted line; -·-·-), 10 (dashed line; ------) and 30 (solid line; —) min. (A) Unmodified HO-1, (B) HO-1 acetylated in the absence of CPR, (C) HO-1 acetylated in the presence of CPR. All reaction mixtures contained 5 μM heme-HO-1 complex, 40 nM CPR and 25 μM NADPH in 0.1M potassium phosphate buffer (pH 7.4).