Table 1.
Identification of the positions of the lysine residues of HO-1 acetylated in the presence or absence of reductases
| Observed mass (m/z) | Peptide | Peptide sequence | Acetylated lysine | Without reductasea | With CPRa | With BVRa |
|---|---|---|---|---|---|---|
| 2119.891 | 1-18 | MERPQLDSMSQDLSEALK | 18 | yes | yes | yes |
| 1124.671 | 19-27 | EATKEVHIR | 22 | yes | yes | yes |
| 1105.643 | 36-44 | NFQKGQVSR | 39 | yes | yes | yes |
| 2783.232 | 45-67 | EGFKLVMASLYHIYTALEEEIER | 48 | yes | yes | yes |
| 2257.072 | 68-85 | NKQNPVYAPLYFPEELHR | 69 | yes | yes | yes |
| 1249.676 | 137-148 | YLGDLSGGQVLK | none | yes | yes | yes |
| 1377.802 | 137-149 | YLGDLSGGQVLKK | none | no | yes | no |
| 1419.823 | 137-149 | YLGDLSGGQVLKK | 149 | yes | no | yes |
| 1902.010 | 137-153 | YLGDLSGGQVLKKIAQK | 149 and 153 | yes | no | yes |
| 896.596 | 178-183 | FKQLYR | 179 | yes | yes | yes |
| 1627.821 | 186-198 | MNTLEMTPEVKHR | 196 | yes | yes | yes |
a
Yes and no indicate whether or not the corresponding peptide (the 3rd column in the same line) was detected.