Table 1.
Simulated and recovered thermodynamic parameters
| Parameter | Simulated | Median ± S.E. | Mean ± S.E. |
| N2 ⇌ 2I | |||
| ΔG0(0) | 14.0 | 13.7 ± 1.0 | 14.2 ± 1.1 |
| CM | 4.2 | 4.3 ± 0.3 | 4.2 ± 0.4 |
| 2I ⇌ 2U | |||
| ΔG0(0) | 11.0 | 11.6 ± 4.4 | 14.0 ± 4.9 |
| CM | 4.0 | 4.0 ± 0.1 | 4.0 ± 0.1 |
Thirty-two simulated fluorescence data sets with random errors added were analyzed according to procedures outlined in Materials and Methods. Medians and means of estimates of thermodynamic parameters recovered by analyzing the 32 data sets are listed together with the parameters with which the data were simulated. Units for ΔG0 (0) are in units of kCal mole−1; CM is in units of molar concentration.