Figure 3.

SDS PAGE analysis of the purification of the A. nidulans AROM protein from E. coli cell lysates. The proteins present at each stage in the purification protocol are shown. Lane 1, boiled codon plus cells containing pEKA15; lane 2, clarified supernatant postsonication; lane 3, pellet from the 55% (NH₄)₂SO₄ precipitation; lane 4, sample from the phenyl-Sepharose pool; lane 5, sample from the sephacryl pool; lane 6, sample from the hydroxyapatite pool; lane 7, sample from the DEAE FPLC pool; lane 7, molecular mass markers of 45 kDa (ovalbumin), 66 kDa (bovine serum albumin), and 97 kDa (Phosphorylase B).