Table 3.

The sequences of the oligonucleotides used in the site-directed mutagenesis of dehydroquinate synthase

Mutation	Mutagenic oligonucleotide	Nonmutagenic oligonucleotide
H275L	GAC TCT ATT GGC CTG GCC ATT GAA GCC	GAC CCC AGT TCA AAA GGT TCC
K152A	TCG ATC GGC GGG GCA ACT GCC ATC GAC	TGA ATC TAC CAT GGC CAG AAG
R130K	TCC ACC TAC ATG AAA GGT GTC CGT TAT	AGC GAC GAA TCC TGT CAG ATC
R130A	TCC ACC TAC ATG GCT GGT GTC CGT TAT	AGC GAC GAA TCC TGT CAG ATC
R264A	GAG GGG GGT CTC GCA AAC CTT TTG AAC	ACG TTC GTC TGC TGA GAC AAC

The sequences of the oligonucleotides used in the site-directed mutagenesis are shown written 5′ to 3′.