Figure 3.

The ensembles of auxilin J-domain structures docked to the Hsc70 ATPase domain. The set of docked J-domains with interfaces most consistent with experiment, the “good” set, is shown on the left (A). The good set has the HPD-loop and preceding helix of the J-domain most fully inserted into the lower cleft. Those docked structures not fully consistent with experiment, the “nonconverged” set including the controls, are shown on the right (B). The three DOT (D1–3) and three ZDOCK (Z1–3) docking sites are shown in (C). The Z3 docking site corresponds to the experimentally determined lower cleft binding site. The ATPase domain is magenta, shown with a bound nucleotide in green.