Figure 3.

Expression of β1,4GalNAc-T gene in testis. (A–C) In situ hybridization of β1,4GalNAc-T gene in wild-type testis. (A) Hematoxylin/eosin staining of the testis from a wild-type mouse ×140. (B) With digoxigenin-labeled RNA probe, in situ hybridization was performed as described in Materials and Methods. (×140. (C) High magnification (×560) of B. (D) Total RNA was extracted from testes of ≈2- to 8-week-old wild-type mice and 20 μg each was applied for Northern blotting. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA was used to hybridize the same membrane as a control.