Figure 4.

Expression of gangliosides in testis. (A) TLC of acidic glycosphingolipids from testis. St., bovine brain ganglioside mixture as a standard. Acidic glycolipids were separated by TLC with a solvent (chloroform/methanol/0.2% CaCl₂, 55:45:10). Resorcinol spray was used for detection. (B) Profiles of acidic glycosphingolipids in nongerm cells. Nongerm cells from testis were cultured primarily after elimination of germ cells, then metabolically labeled with [¹⁴C]glucosamine and analyzed for acidic glycolipids by TLC. Solvent for TLC was as in A.