Figure 5.

Distribution of gangliosides in testis. (A–C) Cholera toxin staining. (A) Staining in wild-type testis. (B) No staining of mutant testis. (C) Staining after neuraminidase treatment of wild-type testis for detection of the complex gangliosides. (D) GD3 expression in mutant testis revealed by anti-GD3 mAb R24. Testis of wild-type mice did not show definite staining (data not shown). (E–J) Immunohistostaining with mAbs specific for GD1a (E, F), GT1b (G, H), and GD1b (I, J). Samples from wild-type (E, G, I) and mutant testis (F, H, J) were compared.