Figure 6.

Hormonal changes and their effects in β1,4GalNAc-T mutant mice. Black bars represent wild type and white bars homozygous mutant (A–F). Ten- to 12-week-old mice were analyzed. (A) Testosterone levels in serum. Note marked reduction in serum testosterone level in the mutant (21.0 ± 4.10 ng/dl) in contrast to the wild type (386.63 ± 111.90 ng/dl). ∗, P < 0.005. (B) Testosterone in testis. In testis, the amounts of total testosterone are comparable, and the amount per weight is somewhat higher in the mutant. ∗∗, P < 0.05. (C) Production of testosterone in cultured testicular cells with or without human chorionic gonadotrophin stimulation in vitro. (D) Serum FSH and LH levels in wild-type and mutant mice. (E) Comparison of muscle size. Circumferences of the gastrocnemius muscles and weights of parts between the knee joints and Achilles tendon were measured. ∗∗, P <0.05. (F) Neuron density at the preoptic area was examined by serial cross-sections, as described (34). Ordinate is unit of pixels. ∗, <0.005.