Figure 8.

Ganglioside–testosterone interaction. [¹⁴C]testosterone was applied to a poly(vinylidene difluoride) membrane on which individual glycolipids had been blotted. Binding and washing were performed as described in Materials and Methods. (A) Each lane contained 1.6 (left) and 0.4 (right) nmol of glycolipids. (B) Relative binding of testosterone to 0.4 nmol of each ganglioside is given as a percentage of the GT1b value (an average of three experiments). (C) Specific inhibition of testosterone binding to gangliosides by unlabeled testosterone. [¹⁴C]testosterone was applied to a poly(vinylidene difluoride) membrane, as in A, with individual inhibitors. Similar experiments with wide range of gangliosides were repeated 3 times, and a representative result only for testosterone and estriol is presented as percent inhibition.