Figure 5.

Flexibility in the crystal. Shown is molecule B of the 5′NT–O1 structure, color-coded according to the B-values from 30 Å to 100 Å (cf. color-code bar in inset). (A) The Cα atoms are depicted as thermal ellipsoids. The ellipsoids are derived from rigid-body movement of the two domains described by the libration tensors. The domain-bridging disulfide bond is shown in yellow as a stick model. (B) The molecule is shown as a Cα-trace. The rotation axis between chain A and chain B of the 5′NT–O1 structure is shown in black (cf. Fig. 3 ▶). The libration tensor of the C-terminal domain is shown in purple. The engineered disulfide bridge is shown in yellow. (C) Same as in B, but the figure is rotated by 90°.