Table 1.
Effect of denatured AK concentration on refolding
| k × 10−4 (s−1) | Relative amplitudes of the different phases (%) | ||||
| [Protein], μM | k1 | k2 | k3 | Rf | Rs |
| 1.2 | — | 82.82 | 1.19 | 86 | 14 |
| 3.2 | — | 83.14 | 1.22 | 87.5 | 12.5 |
| 64 | — | 83.31 | 1.26 | 89.5 | 10.5 |
(k) apparent kinetics constant; “—” not determined; Rf and Rs represented the relative amplitude of fast and slow phases in the refolding process, respectively.