Figure 2.

Characterization of amyloid fibrils from the various proteins formed at pH 2.0. (Left) EM images of fibrils from (A) hen lysozyme, (B) I55T lysozyme, (C) turkey lysozyme, and (D) human α-lactalbumin, incubated at pH 2.0 using conditions described in Materials and Methods. The scale bar represents 200 nm. Characterization of the fibril solutions by Congo red (CR) absorption (center) and far-UV CD (right) are also shown for the same proteins: (squares) CR in buffer; (triangles) CR in the presence of fibrils; and CD spectra of freshly dissolved protein (squares) and protein fibrils (triangles).