FIGURE 5.

Immobilization of PBA-modified oligonucleotides on SHA-affinity membranes. Radiolabeled PBA-modified or -unmodified oligonucleotides (1000 nM in 30 μL 10× SSC) were bound to SHA-affinity membranes for 15 min at room temperature. After binding, membranes were washed, and bound oligonucleotides were released by adding 60 μL 1 mM Versalinx Nucleic Acid Releasing Reagent in distilled water and incubating at 37°C for 10 min. Bound and released oligonucleotides were determined by scintillation counting of membrane, flow-through, and input fractions, and are expressed as a percentage of input fractions.